In addition, the similarity of the BLAST 16S rRNA sequence and biochemical tests showed that these strains in GenBank were closely linked to LAB. The genus Pediococcus comprises homofermentative coccoidal lactic acid bacteria that occur in pairs or tetrads through division in two planes. The species diversity was observed because eight species were also identified: P. pentosaceus (24.2%), Lc. This indicates that species of LAB predominate the microbial composition of traditionally prepared dry starters in Asia, including the Eastern Himalayas. Species often form black colonies to olive green colonies on initial isolation. Also, the organisms presented tolerance to 6.5% NaCl and 0.3% bile salt. 3. inopinatus, Ped. Morphological and biochemical identification: ... 90, 81, 96, 98, 94, 93 and 94% DNA sequence identity of known species Lactobacilli and Pediococcus registry entries . garvieae (15.2%), Lc. We report the presence of Pediococcus species with probiotic properties from betel. acidilactici, Ped. Urease-negative. pentosaceus and Ped. Identification of Pediococcus and Tetragenococcus species. The colonies are convex, circular, and have a shiny, smooth appearance. parvulus, Ped. lactis (15.2%), E. The genus consists of seven species: Ped. The black pigment fades quickly upon exposure to oxygen and within a week some colonies take on a mustard yellow pigmentation. damnosus, Ped. Several species of LAB in Cambodian dombea were also reported: Weissella cibaria, Lactobacillus plantarum, Lactococcus lactis, Pediococcus pentosaceus, and Enterococcus durans (Ly et al., 2018). Enterococci can be quickly differentiated from Pediococcus and Leuconostoc using simple biochemical tests. The biochemical characteristics of the most recently identified strains of A. viridans (28 strains) E. avium (28 strains) and S. uberis (9 strains, all nonhuman) were compared to the 28 strains of G. sanguinus. dextrinicus, Ped. The biochemical and phylogenetic analyses revealed that most of the characterized LAB belong to the genera Lactobacillus, Lactococcus, Pediococcus, Enterococcus and Leuconostoc. lactis subsp. Gram-positive rods, not inhibited by the Vancomycin disk. determination of the biochemical profile of the strain and its identification. RESULTS AND DISCUSSION Bacteria isolated from the foregut, midgut and hindgut of Tilapia guineensis were identified as pediococcus pentosaceus2 and pediococcus pentosaceus1 by observing their colony morphology, urinaeequi ( Anonymous 1999).One Pediococcus species, Ped. Additionally, infection control methods may vary greatly between vancomycin resistant organisms, so informing the clinical team about the organism identification rapidly is imperative. Table 1 Morphological and biochemical identification of isolates Isolates Form CO 2 Growth temperature NaCl 6.5% Growth pH Genus* 10°C 37°C 45°C 4.4 9.6 M101 Cocci - - + + - + - Pediococcus M102 Cocci - - + + + - - Lactococcus M103 Cocci - - + + - + - Pediococcus KEY BIOCHEMICAL REACTIONS Nitrate is not reduced. The isolated organisms were subjected to preliminary biochemical analysis and exhibited growth at 37°C and pH 6.7 with fermented glucose, sucrose and lactose without the evolution of CO2.

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